Abstract
ABSTRACT Peppers of the genus Capsicum are of great socioeconomic importance, being pungency trait their main attraction. Pungency characterization, genetic distance estimates and population structure analysis of the accessions belonging to germplasm banks are important for parent selection which allows to obtain superior progenies. Therefore, the aims of this study were: i) evaluate 81 accessions of the Capsicum spp. Germplasm Bank of Universidade Federal de São Carlos (BGC-UFSCar) with molecular markers linked to pungency; ii) estimate the genetic diversity among accessions of the BGC-UFSCar using microsatellite markers (SSR); and iii) evaluate the efficiency of these markers in the distinction among species of Capsicum spp. We noticed that pun11 and SNP molecular markers were efficient in predicting the pungent phenotype of BGC-UFSCar accessions in 84.85% and 95.59%, respectively. From a total of 13 amplified microsatellite markers, seven were polymorphic and efficient to discriminate species of Capsicum genus, both through genetic diversity analysis and population structure analysis, which showed three subpopulations. The molecular markers used in this study are useful tools for breeding programs since they were able to characterize and discriminate Capsicum spp. species at DNA level. Information obtained with molecular markers can assist in the selection of contrasting parents for future breeding programs.
Highlights
Pimentas do gênero Capsicum possuem grande importância socioeconômica, sendo a pungência seu principal atrativo
SSR markers are characterized by sequences, called motifs, from one to six nucleotides, which are repeated in tandem, considering that the polymorphism produced consists of the difference in the number of replicates of motifs in each allele (Buso et al, 2016)
Molecular markers associated with pungency in Capsicum spp. had already been described in literature
Summary
Pimentas do gênero Capsicum possuem grande importância socioeconômica, sendo a pungência seu principal atrativo. Os objetivos deste estudo foram: i) avaliar 81 acessos do Banco de Germoplasma de Capsicum spp. da Universidade Federal de São Carlos (BGC-UFSCar) com marcadores moleculares relacionados a pungência; ii) estimar a diversidade genética entre os acessos do BGC-UFSCar por meio de marcadores microssatélites (SSR) e iii) avaliar a eficiência desses marcadores na distinção das espécies do gênero Capsicum. Os marcadores moleculares utilizados no presente estudo são ferramentas úteis para programas de melhoramento, pois foram capazes de caracterizar e discriminar a nível de DNA as espécies de Capsicum spp. Germplasm Bank of Universidade Federal de São Carlos (BGC-UFSCar) with molecular markers related to pungency; ii) analyze the genetic diversity in accessions using SSR markers and iii) evaluate the efficiency of these markers in the distinction among the species of Capsicum spp
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