Abstract
In the present study pearl millet oligosaccharides (PMOs) were extracted at optimized process parameters and further purified by passing through charcoal column and dialysis membrane (500 Da) to obtain a purity >90% with a yield of 4.6% (w/w). The molecular weights of the mixed oligosaccharides (342, 504, 667, 829, 991, 1153, 1316 Da) were confirmed by MALDI-TOF. The non-digestibility assay was performed by acid hydrolysis, salivary and pancreatic α amylases. The results showed a maximum hydrolysis of 0.2516% ± 0.0173, 2.8% ± 0.023 and 3.1% ± 0.11 by gastric acid, salivary and pancreatic α-amylases respectively. A positive prebiotic score, enhanced biofilm formation and capability of producing SCFAs (i.e. acetate, propionate and butyrate) by different strains of lactobacilli in presence of purified oligosaccharides hints towards their prebiotic potential. From the observations we propose that the PMOs can be explored further as nutraceuticals and functional food additives.
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