Abstract
The aim of this study was to clarify degradation characteristics in each tissue of the knee complex of a medial meniscectomy (MMx)-induced knee osteoarthritis (KOA) animal model using classical methods and an alternative comprehensive evaluation method called contrast-enhanced X-ray micro-computed tomography (CEX-μCT), which was developed in the study. Surgical MMx was performed in the right knee joints of five male Wistar rats to induce KOA. At four weeks post-surgery, the synovitis was evaluated using quantitative polymerase chain reaction (qPCR). Degradations of the articular cartilage of the tibial plateau were evaluated using classical methods and CEX-μCT. Evaluation of the synovitis demonstrated significantly increased expression levels of inflammation-associated marker genes in MMx-treated knees compared with those in sham-treated knees. Evaluation of the articular cartilage using classical methods showed that MMx fully induced degradation of the cartilage. Evaluation using CEX-μCT showed that local areas of the medial cartilage of the tibial plateau were significantly reduced in MMx-treated knees compared with those in sham-treated knees. On the other hand, total cartilage volumes were significantly increased in MMx-treated knees. On the basis of the findings of this study, the method could be relevant to study new treatments in KOA research.
Highlights
The prevalence of osteoarthritis (OA) in humans has been rapidly increasing owing to an aging population and a rise in obesity rates [1]
Oypalomin allowed for plateau was stained using various concentrations of Oypalomin the depiction of articular cartilage from the medial tibial plateau
Oypalomin could allow for visualization of the articular cartilage and provided between the bone and cartilage (Figure 2A)
Summary
The prevalence of osteoarthritis (OA) in humans has been rapidly increasing owing to an aging population and a rise in obesity rates [1]. OA is characterized by chronic pain, dysfunction of joint, and tissue degradation of joint cartilage and subchondral bone with synovitis [2]. Causes high morbidity and is recognized as the most common type with a prevalence of 80% among all OA [1]. Advanced KOA causes severe pain and significant limitations in daily life [3]. KOA, if the symptoms are not relieved by exercise and intra-articular injection of hyaluronic acid, knee replacement using an artificial knee joint is often performed [3]. Studies on the development of drugs to treat KOA are often performed using animal models
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