Abstract
Methods HIV-1 Tat exon 1 and Vpr were amplified from the DNA isolated from blood of HIV infected patients and cloned. Clones were got sequenced and aligned against reference sequences using CLUSTAL W. Sim plot analysis was done for recombinants. Their expression was accessed by transfection of HEK 293T cells with myc fusion clones of variants and western blotting using anti-myc antibody. The variant Tat clones were cotransfected with LTR-luc to investigate their LTR transactivation potential by dual luciferase reporter assay.
Highlights
HIV-1 Tat & Vpr are multifunctional and involved in transactivation, cell cycle regulation, MHC-1 modulation etc
HIV-1 Tat exon 1 and Vpr were amplified from the DNA isolated from blood of HIV infected patients and cloned
One Vpr variant had a frameshift towards Cterminus
Summary
HIV-1 Tat & Vpr are multifunctional and involved in transactivation, cell cycle regulation, MHC-1 modulation etc. Like other HIV genes, Tat and Vpr are subject to variation. Recombination frequency is higher in the first exon of Tat and Vpr. Recombination frequency is higher in the first exon of Tat and Vpr Characterization of these variants is the subject of the present study
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