Abstract
.Significance: Assessment of disease using optical coherence tomography is an actively investigated problem, owing to many unresolved challenges in early disease detection, diagnosis, and treatment response monitoring. The early manifestation of disease or precancer is typically associated with subtle alterations in the tissue dielectric and ultrastructural morphology. In addition, biological tissue is known to have ultrastructural multifractality.Aim: Detection and characterization of nanosensitive structural morphology and multifractality in the tissue submicron structure. Quantification of nanosensitive multifractality and its alteration in progression of tumor.Approach: We have developed a label free nanosensitive multifractal detrended fluctuation analysis(nsMFDFA) technique in combination with multifractal analysis and nanosensitive optical coherence tomography (nsOCT). The proposed method deployed for extraction and quantification of nanosensitive multifractal parameters in mammary fat pad (MFP).Results: Initially, the nsOCT approach is numerically validated on synthetic submicron axial structures. The nsOCT technique was applied to pathologically characterized MFP of murine breast tissue to extract depth-resolved nanosensitive submicron structures. Subsequently, two-dimensional MFDFA were deployed on submicron structural en face images to extract nanosensitive tissue multifractality. We found that nanosensitive multifractality increases in transition from healthy to tumor.Conclusions: This method for extraction of nanosensitive tissue multifractality promises to provide a noninvasive diagnostic tool for early disease detection and monitoring treatment response. The novel ability to delineate the dominant submicron scale nanosensitive multifractal properties may also prove useful for characterizing a wide variety of complex scattering media of non-biological origin.
Highlights
Disease progression in living tissues expected to exhibit nanosensitive structural alteration at the submicron scale
We have simulated nanosensitive optical coherence tomography (nsOCT) in synthesized volume (1024 × 1024 × 400 voxels) phantom composed of randomized submicron structures throughout the volume
Hurst exponent [hðq 1⁄4 2Þ] in synthetic and nsOCT constructed en face is almost equal in values confirms our capability to detect submicron scale structural correlation within a complex tissue sample
Summary
Disease progression in living tissues expected to exhibit nanosensitive structural alteration at the submicron scale. In an initial ex vivo study on murine tissue, we found interesting change in depth-resolved nanosensitive multifractality in submicron structures after tumor formation in breast tissue samples This method for extraction of nanosensitive tissue multifractality promises to develop a noninvasive diagnosis tool for the detection of cancer development. This newly developed method offers exciting depth-resolved ultrastructural detection for better treatment and monitoring if there is a tumor response to treatment
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
