Characterization of muscle actin genes in Drosophila virilis reveals significant molecular complexity in skeletal muscle types.

Abstract

Actin is a ubiquitous and highly conserved eukaryotic protein required for cell motility and locomotion. In this manuscript, we characterize the four muscle actin genes of the insect Drosophila virilis and demonstrate strong similarities between the D. virilis genes and their homologues in Drosophila melanogaster; intron locations are conserved, and there are few amino acid differences between homologues. We also found strong conservation in temporal expression patterns of the muscle actin genes--the homologues of the D. melanogaster genes Act57B and Act87E are expressed throughout the life cycle, whereas the other two D. virilis genes, homologous to Act79B and Act88F are specific to pupal and adult stages. In situ hybridization revealed that each D. virilis gene is expressed in a unique pattern in the muscles of the thorax and abdomen. These muscle-specific patterns of actin isoforms suggest a greater physiological diversity for the adult muscles of insects than has been appreciated to date from their categorization into fibrillar, tubular (non-fibrillar) and supercontractile muscle types.