Characterization of MS2 Bacteriophage by Integrated Virus Detection System (IVDS) Physical Counting Methodology.

Abstract

Abstract : A new physically based methodology - The Integrated Virus Detection System (IVDS) - was used to characterize a high concentration, 10.2 mg protein/ml, sample preparation of MS2 Bacteriophage with a reported 10(exp 14) pfu/ml (DPM14) virus count in a common TNME buffer. Virus counts were made using the IVDS instrument following serial dilution. Results indicated virus counts of 1.5 x 10(exp 5) for the neat sample (DPM14), followed by 6.5 x 10(exp 4) viruses (DPM13), 1.2 x 10(exp 4) viruses (DPM12), 9.3 x 10(exp 2) viruses (DPM11), 88 viruses (DPM10), and 5 viruses (DPM9), respectively. Lower concentrations display a consistent multiplier and were consistent with target dilutions. Increases in virus concentration appear to decrease the multiplier. Variation is considered to be due to aggregation. Results demonstrate a consistent and simple-to-use methodology. Results further indicate that the IVDS instrument can be used for characterization of other virus preparations with equal ease and similar results.