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Abstract
Chemotactic factor was produced in vitro by antigen or mitogen stimulation of mouse spleen cells. Activity was eluted from Sephadex columns in a major peak at 38,000 daltons. Small amounts of activity sometimes occurred in the 13,000 molecular weight region. Total recovery in the eluted fractions ranged from 30 to 60% of the applied material. The molecular weight of mouse lymphocyte-derived chemotactic factor (LDCF) is different from that of human, guinea pig, and chicken, all of which have reported molecular weights of 12,500 to 15,000. Mouse LDCF was eluted from DEAE columns at a specific conductance of about 15 mmho/cm, which indicates an apparent net charge different from chemotactic factor produced by stimulated human peripheral blood leukocytes. Thus, among lymphocyte-derived chemotactic factors hitherto described, the 38,000 MW molecule produced by mouse spleen cells is unique.
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