Year-end Sale % OFF 
Abstract
Summary Monoclonal antibodies against cardiac myosin from chicken (18d embryos) and rabbit were produced in vitro by fusion of mouse myeloma cells (P3-X63-Ag8) with spleen cells from immunized BALB/c mice and Lewis rats. Three antibodies were examined and found to react with different antigenic determinants in the myosin heavy chain subunit. Antibody RCM-79 reacted specifically with heavy meromyosin. Antibodies CCM-13 and RCM-79 reacted with equal affinities to cardiac myosin from chicken, rabbit, rat, and man, but with lower affinities to skeletal muscle myosins from chicken and rabbit. Antibody CCM-31A reacted only to chicken cardiac myosin and not to other cardiac or skeletal myosins tested. Our results show that monoclonal antibodies can be produced that are either specific for sequences common to all of the cardiac myosins examined or unique to the immunizing type.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
