Abstract
Isokinetic sucrose gradients were used for the separation of different strains of murine sarcoma viruses (MuSV) from their associated helper leukemia virus (MuLV). Two viral-specific peaks as determined by reverse transcriptase assay were obtained after a mixture of MuSV produced by MuSV349 cells and Moloney MuLV were centrifuged in an isokinetic gradient. The slower sedimenting peak fraction was shown to contain MuSV by the presence of RNA species which are smaller in size (∼2.2 × 10 6 daltons) than the genomic size of MuLV RNA, a protein gel electrophoresis profile similar to that of MuSV, the ability of the virus to form foci in TB and NRK cells, and its inability to produce syncytia in XC assay. The faster sedimenting peak was shown to contain MuLV by the presence of mainly MuLV genomic size RNA (∼3.2 × 10 6 dalton), a protein gel electrophoresis profile similar to that of MuLV, and the ability of the virus to produce syncytia in the XC assay.
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