Abstract
Previous studies1, 2, 3, 4, 5, 6 indicate the presence, in at least certain tissues of high cancer strain mice, of a substance or substances whose presence is essential to spontaneous mammary carcinoma. In these studies lactating mammary tissues inclusive of their contained milk have been brought into suspension and partial solution by micro-homogenization and the resulting fluid separated into several fractions by the use of an ultracentrifuge.Procedure. Fostered female C3H mice 1 month old were employed as test animals. Lactating breast tissues were obtained immediately after death by etherization, from high cancer C3H and A strain mice. The material was immediately homogenized with addition of between one and two times its volume of sterile distilled water. The resulting fluid was spun in the centrifuge at 15,000 rpm, giving a maximum accelerational force of 15,000 x g, for 30 minutes. Three layers resulted, a fatty top layer, designated as the fat fraction, a middle aqueous layer, the first supernatan...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
