Abstract
Seven microsatellite loci were isolated and characterized from a microsatellite enriched genomic library for the stingless bee Scaura latitarsis. Primers were tested in 12 individuals. The number of alleles per locus ranged from 3 to 6 (mean = 4.29) and observed heterozygosity ranged from 0.00 to 0.75 (mean = 0.40). Cross-species tests showed successful amplification for Scaura atlantica, Scaura longula, Scaura tenuis, Schwarzula timida, Schwarziana quadripunctata, Plebeia droryana, and Plebeia remota.
Highlights
Scaura latitarsis is small (4.0 mm) and black stingless bee which nidifies in arboreal termite nests of Nasutitermes (Michener 2007)
Microsatellites have been the nuclear marker most used in population genetic studies
The microsatellite-enriched genomic library was constructed according to Billotte et al (1999) with some modifications (Brito et al 2009)
Summary
Scaura latitarsis is small (4.0 mm) and black stingless bee which nidifies in arboreal termite nests of Nasutitermes (Michener 2007). The species is widely distributed in tropical South America, with records in the following countries: Colombia, Venezuela, Copyright Flávio O. The large geographic distribution and the well reported female philopatric behavior in stingless bees (Nogueira-Neto 1954, Brito and Arias 2010, Francisco and Arias 2010) suggest that S. latitarsis populations should be highly differentiated. Population studies apply combined molecular markers of nuclear and cytoplasm origins.
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