Abstract
Induced pluripotent stem cells (iPSCs) technology provides a powerful means to generate and regenerate unlimited pluripotent stem cells directly from body tissue cells. Stem cells from apical papilla (SCAP) and Dental pulp stem cells (DPSCs) are present in ‘cell-rich zones’ within the dental pulp region, which are capable of regenerating pulp and dentin tissues in vivo. In this study, we investigated the difference of miRNAs expression in SCAPs and DPSCs before and after the reprogramming. Using miRNA microarray, 134 and 265 differentially expressed miRNAs in DPSCs- and SCAP-iPSCs were up-regulated compared to these before reprogramming. 117 specific miRNAs with enhanced more than 2-fold were identified in both DPSCs- and SCAP-iPSCs. Among the co-regulated miRNAs, miR-19a-3p, miR-92b-3p and miR-130b-3p showed the maximum difference, which had involvement in the cell cycle, TGF beta signaling pathway and epithelial mesenchymal transition. Using qRT-PCR analysis, the expression of miR-19a-3p, miR-92b-3p and miR-130b-3p indicated substantial increases in DPSCs-iPSCs and SCAP-iPSCs. The findings suggest that miRNAs play a part in the difference between DPSCs-iPSCs and DPSCs, as well as between SCAP-iPSCs and SCAP. The variation of miRNA expression in reprogrammed dental-derived pluripotent stem cells revealed different characteristics induced by iPSC generation.
Highlights
In the past ten years, the establishment of induced pluripotent stem cells has been as one of the breakthroughs in the field of stem cell research [1, 2]
Dental pulp stem cells (DPSCs) and Stem cells from apical papilla (SCAP) isolated from third molars (Fig 1) were culture in medium consisting of α-MEM (Gibco/Invitrogen, Grand Island, NY, USA), 10% fetal bovine serum (Thermo Scientific), 1% Glutamax (Gibco/Invitrogen, Grand Island, NY, USA) and 0.5% Penicillin/Streptomycin ((Invitrogen)
The flow cytometric analyses showed that DPSCs and SCAP were both negative for the haematopoietic surface markers of CD34 and CD45
Summary
In the past ten years, the establishment of induced pluripotent stem cells (iPSCs) has been as one of the breakthroughs in the field of stem cell research [1, 2]. This breakthrough discovery provides a powerful means to generate and regenerate unlimited pluripotent stem cells directly from body tissue cells [3]. Like embryonic stem cells (ESCs), iPSCs share the majority properties with embryonic stem cells, such as morphology, differentiation, DNA methylation, gene expression, unlimited self-renewal ability and potential to generate any differentiated cell type (pluripotency) [4]. A number of co-expressed miRNAs clusters, such as miR-302-367, and miR-290, were disclosed in embryonic stem cells, iPS cells and somatic cells [8]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
