Abstract
Pasteurella multocida is the pathogenic agent for a variety of severe diseases in livestock, including rabbits. MicroRNAs (miRNAs) participate in the immune response to the pathogen. Distinct miRNA expression patterns were explored in rabbit lung by small-RNA deep sequencing to assess dysregulated miRNAs during P. multocida infection. Totally, 571 miRNAs were screened, of which, 62 were novel, and 32 exhibited differential expression (DE). Of the 32 known DE-miRNAs, 13 and 15 occurred at 1 day and 3 days post-infection (dpi); and ocu-miR-107-3p and ocu-miR-29b-5p were shared between the two time points. Moreover, 7,345 non-redundant target genes were predicted for the 32 DE-miRNAs. Putative target genes were enriched in diverse GO and KEGG pathways and might be crucial for disease resistance. Interestingly, upregulation of ocu-miR-29-5p suppresses P. multocida propagation and downregulates expression of epithelial membrane protein-2 (EMP2) and T-box 4 (TBX4) genes by binding to their 3′ untranslated region in RK13 cells. Thus, ocu-miR-29-5p may indirectly inhibit P. multocida invasion by modulating genes related to the host immune response, such as EMP2 and TBX4.
Highlights
Pasteurella multocida (P. multocida) is a pathogenic Gram-negative bacterium that frequently infects the respiratory tract of most livestock and causes significant economic loss worldwide [1, 2]
The results indicated that expression levels of ocu-miR-29b5p in all four tissues were markedly elevated after P. multocida microRNA Profiles in Pasteurella multocida challenge
We first report a comprehensive assessment of rabbit miRNA expression in the course of P. multocida infection
Summary
Pasteurella multocida (P. multocida) is a pathogenic Gram-negative bacterium that frequently infects the respiratory tract of most livestock and causes significant economic loss worldwide [1, 2]. 16 serotypes are recognized using lipopolysaccharide (LPS) antigens [3, 4]. Rabbits can become infected with P. multocida immediately after birth. The infection causes rhinitis in the upper respiratory and pneumonia in the lower respiratory tracts [1, 5]. Prevalence rates as high as 94% are reported for P. multocida, and, the majority of adult rabbits are microRNA Profiles in Pasteurella multocida recognized as carriers [6]. P. multocida can cause human infections via animal bites and/or scratches [2, 7, 8]
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