Characterization of Microbial Communities from the Alimentary Canal of Typhaea stercorea (L.) (Coleoptera: Mycetophagidae).

Abstract

Simple SummaryHairy fungus beetle, Typhaea stercorea, is a secondary post-harvest pest of stored grains that thrives by feeding on mytoxigenic fungi. Bacterial communities residing in the alimentary canal of most insects contribute to their host’s development. While there are many examples, little is known about the role of bacterial communities in the alimentary canal of T. stercorea. The objectives of this study were to (1) characterize the microbial communities residing in T. stercorea and (2) compare the microbial compositions of field-collected and laboratory-reared populations. In this study, we were able to identify bacterial communities that possess mycolytic properties and track mark changes in the microbiota profiles associated with development. The genus Pseudomonas was enriched in T. stercorea larvae compared to adults. Furthermore, field-collected T. sterocrea adults had a lower species richness than both larva and adult laboratory-reared T. sterocrea. Moreover, the gut microbial compositions of field-collected and laboratory-reared populations were vastly different. Overall, our results suggest that the environment and physiology can shift the microbial composition in the alimentary canal of T. stercorea.The gut microbiomes of symbiotic insects typically mediate essential functions lacking in their hosts. Here, we describe the composition of microbes residing in the alimentary canal of the hairy fungus beetle, Typhaea stercorea (L.), at various life stages. This beetle is a post-harvest pest of stored grains that feeds on fungi and serves as a vector of mycotoxigenic fungi. It has been reported that the bacterial communities found in most insects’ alimentary canals contribute to nutrition, immune defenses, and protection from pathogens. Hence, bacterial symbionts may play a key role in the digestive system of T. stercorea. Using 16S rRNA amplicon sequencing, we examined the microbiota of T. stercorea. We found no difference in bacterial species richness between larvae and adults, but there were compositional differences across life stages (PERMANOVA:pseudo-F(8,2) = 8.22; p = 0.026). The three most abundant bacteria found in the alimentary canal of the larvae and adults included Pseudomonas (47.67% and 0.21%, respectively), an unspecified genus of the Enterobacteriaceae family (46.60 % and 90.97%, respectively), and Enterobacter (3.89% and 5.75%, respectively). Furthermore, Pseudomonas spp. are the predominant bacteria in the larval stage. Our data indicated that field-collected T. stercorea tended to have lower species richness than laboratory-reared beetles (Shannon: H = 5.72; p = 0.057). Furthermore, the microbial communities of laboratory-reared insects resembled one another, whereas field-collected adults exhibited variability (PERMANOVA:pseudo-F(10,3) = 4.41; p = 0.006). We provide evidence that the environment and physiology can shift the microbial composition in the alimentary canal of T. stercorea.