Abstract
Summary A total of 24 strains of Methylophaga , marine methylotrophic bacteria, were characterized by rRNA gene restriction patterns (ribotypes), after cleavage of total DNA with Eco RI or Hin dIII restriction endonuclease. Manual ribotyping and the RiboPrinter® Microbial Characterization System were used. The two genomic DNA groups could be differentiated each by ribotyping. Our study showed that ribotyping could characterize Methylophaga species and identify unknown isolates. All results were confirmed by DNA/DNA hybridization. Methylophaga sulfidovorans species was confirmed to be a third species by ribotyping and DNA/DNA hybridization.
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