Abstract

Effect of perturbation factors on phase transition metastable positions of whole milk (4% fat content) and their influence on microbial destruction characteristics of non-pathogenic Escherichia coli inoculated in milk subjected to high pressure low temperature treatment were evaluated using a specially developed high pressure (HP) cooling system. Initially, the phase transition data of milk transitioning through the metastable phases were obtained and fitted successfully using Simon-like models as done in previous studies and polynomial formulas with R2 of 0.997 & 0.996 for ice I, and 0.989 & 0.989 for ice III, respectively. The phase transition position of milk was explored with 5% and 10% sodium chloride solution as perturbation sources, respectively. Results showed that the 5% sodium chloride solution can reduce the transition pressure of milk by 43 MPa and increase the transition temperature by 4.1 °C, so that the milk can achieve phase transition at lower pressure and higher temperature. Phase transition microbial destruction was characterized by discontinuity, mutation and segmentation when the phase transition pressure interval 250– 300 MPa was carefully refined. The inactivation amount of E. coli before the phase transition (250 MPa) was 1.11 log and the phase transition process itself brought an additional 1.26 log destruction of E. coli population in milk. Industrial relevanceHigh pressure low temperature (HPLT) phase change kinetics were employed to enhance microbial destruction. HPLT was established based on a self-cooling unit positioned inside conventional HP chamber offering opportunities for scale up and commercialization. The effectiveness of HPLT phase transition for Escherichia coli destruction was demonstrated. The related research in metastable state provides a reference point for commercial application of high-pressure-low-temperature technology for microbial destruction and quality enhancement.

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