Abstract
The application of capillary electrophoresis to the separation and detection of compounds of medical interest is described. A proton-induced X-ray emission (PIXE) detector was used for metal-specific and sensitive detection. The detected X-rays are generated inside the capillary by an accelerated proton beam. The application of this novel detection mode in addition to common UV detectors is demonstrated for different complexes of gadolinium with polyaminopolyacetic acids. Using the PIXE detector lower limits of detection have been achieved which could be convincingly demonstrated by a comparison with UV detection in the direct and indirect detection mode. Furthermore, kinetic experiments investigating the decomposition of the Re(III)-HEDP (1-hydroxyethylidene diphosphonic acid) boon seeking agent were discussed. Finally, metallothioneins (MTs) from rabbit liver and horse kidney have been separated and up to six isoforms were completely or partially resolved. Copper, zinc and cadmium, attached to the isoforms, have been detected simultaneously.
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