Abstract
This study investigates the mechanism of magnesium (Mg2+) transport in erythrocytes of rat, cow, pig and human by the method of atomic absorption spectroscopy. Erythrocytes of rat, pig, and cow, which were loaded with Mg2+ employing 6μM A23187 and 12 mM MgCl2, released Mg2+ in a time-dependent manner with maximal efflux occurring within 40–50 min. Pretreatment of preloaded erythrocytes with either 10-3 amiloride, or replacement of extracellular sodium with N-methyl-D-glucamine (NMDG), resulted in a significant inhibition of Mg2+ release compared to erythrocytes incubated in normal buffer. Conversely, in human erythrocytes both amiloride and NMDG had no effect on Mg2+ efflux compared to erythrocytes incubated in normal buffer. The results indicate that Mg2+ efflux from erythrocytes of rat, pig, and cow are Na+ dependent in contrast to efflux from human erythrocytes which appears to be non-Na+ dependent.
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