Abstract

Rhizoctonia solani Kühn is the causal pathogen of tobacco target spot, a serious fungal disease of tobacco that severely impairs yield and quality in northeast China. The objective of this study was to characterize isolates ofR. solanifrom tobacco in China. Among 58Rhizoctoniaisolates examined, all of them were multinucleate. Phylogenetic analyses and hyphal anastomosis criteria suggest that the isolates belonged toR. solanianastomosis group (AG) 3. Target spot isolates from Liaoning province formed a single phylogenetic group together with tomato isolates ofR. solaniAG-3 from Japan and are more closely related toR. solaniAG-3 isolates in tomato and potato than that in tobacco from USA. Pathogenicity test for each isolates was fulfilled using a method of inoculating tobacco leaves from plants grown for 8 weeks (cv. NC89).

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