Abstract
The physical grounds for phase-contrast and confocal-reflection microscopies have been used to explain lipid microphase structures by rationalization of the interaction of light with the lipid multilayers. Four types of lipid microphase structuresoily streaks, polygonal arrays, and bubble and striped domainswhich can be characterized by conventional polarizing microscopy were visualized and analyzed in detail by phase-contrast and confocal-reflection microscopies. We find that the images obtained through phase-contrast and confocal-reflection microscopies have even better correlation with lipid alignment models. The complementary nature of polygonal arrays is revealed here by confocal-reflection microscopy. Both confocal-reflection and phase-contrast microscopies were used to characterize the transition processes between striped and bubble domains.
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