Characterization of lipase from lipase-producing bacteria and its application in the reduction of triglyceride content in milk sample

Abstract

Lipases hydrolyse esters of long-chain fatty acids. They have several biotechnological applications such as in the field of food technology, leather, cosmetics, detergents, textiles, oleochemicals, pharmaceuticals and industrial waste. Lipases occur widely in nature in animals, plants and microorganisms; however, in terms of commercial significance, microbial lipases are the most beneficial owing to the ease of production. This study aimed to characterise lipase isolated from lipase-producing bacteria, which were screened on Tributyrin agar to confirm lipase production. Growth parameters of the isolates including their temperature and pH tolerance were determined so that they can be applied for potential industrial uses. The enzymatic activity, pH optimum and temperature optimum of the lipase were determined by the p-nitro phenyl palmitate assay. Finally, the lipase was used to reduce triglyceride content in full-fat buffalo milk.