Abstract
Pituitary cells produce leukotrienes (LTs) and respond to exogenous administration of LTs by releasing gonadotropins. Specific high affinity leukotriene C 4 (LTC 4) binding has been found in membrane preparations of bovine anterior pituitaries. Unlabelled LTC 4 displaced specific [ 3H]LTC 4 binding. Other leukotrienes (LTB 4, LTD 4, LTE 4, LTF 4) did not compete with [ 3H]LTC 4 for binding sites when administered at increasing concentrations together with a constant amount of radioligand indicating that the binding is highly specific for LTC 4. Scatchard analysis of binding data obtained from saturation studies reveavled a single binding site for [ 3H]LTC 4 with a Kd of 8.95 +/− 5.53 mM and a B max of 15.44 +/− 6.93 pmol per mg of membrane protein. Glutathione S-transferase, a possible LTC 4 biding site, did not display activity in the membrane fraction although the two glutathione derivates S-octylglutathione and S-decyglutathione competed with LTC 4 in binding experiments. As leukotrienes are potent stimulators of gonadotropin secretion and modulators of gonadotrophin-releasing homone (GnRH)-induced gonadotropin release it is concluded that leukotrienes may be involved in the signal transduction pathway of GnRH and that they may act via a specific and high affinity receptor.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
