Characterization of l-threonine and l-glutamine transport in murine P388 leukemia cells in vitro. Presence of an N-like amino acid transport system

Abstract

The transport of l-threonine and l-glutamine into murine P388 leukemia cells has been characterized. Threonine appears to be a specific substrate for a Na +-dependent amino acid transport system similar to system ASC of the HTC hepatoma cell. Threonine transport is uninhibited by 2-aminobicyclo[2,2,1]heptane-2-carboxylic acid and α-(methylamino)isobutyric acid, shows a pattern of transport similar to that seen in HTC hepatoma cells over the pH range of 5.5–7.5, and is inhibited by l-serine and l-cysteine. Approximately two-thirds of glutamine transport into P388 cells also appears to enter P388 cells via this ASC-analogous system. However, based upon (a) inhibition studies with threonine (where the K 1 of threonine inhibition of glutamine transport was 7-fold the K m of threonine transport), (b) inhibition analysis of glutamine transport with various amino acids and amino acid analogues, and (c) different patterns of transport between threonine and glutamine over the pH range of 5.5–7.5, approximately one-third of glutamine transport can be attributed to a second Na +-dependent amino acid transport system. This system appears to be similar to the system N of rat hepatocytes. Glutamine and threonine do not appear to enter P388 cells via systems A or L to any significant degree. P388 cells do not appear to exhibit ‘adaptive regulation’ of amino acid transport. Differences in ‘adaptive regulation’ could therefore not be utilized for comparing threonine and glutamine transport.