Abstract
Multidrug-resistant tuberculosis (MDR-TB) is commonly found in Thailand especially in the public health region 5, the Western region of Thailand. This study's aim was to characterize katG, inhA, rpoB and pncA genes in Mycobacterium tuberculosis. One hundred strains of Mycobacterium tuberculosis (MTB) were isolated from sputum samples of MDR-TB risk patients in the laboratory of the Office of Disease Prevention and Control 5th Ratchaburi province, Thailand from January to December 2015. Drug susceptibility testing (DST) was performed using a BACTEC MGIT 960 system. Furthermore, the genes katG, inhA, rpoB and pncA were characterized by DNA sequencing. Of a total of 100 MTB samples which underwent drug susceptibility testing, 42% showed isoniazid (INH) and rifampicin (RIF) resistance, and a further 25% showed INH mono-resistance (25%). The most common gene mutations found using DNA sequencing were katG_Ser315Thr (70%), rpoB_Ser531leu (81%) and pncA_Ile31Thr (84%). The common mutation of pncA_Ile31Thr substitution was detected in 26 of 91 (29%) pyrazinamide (PZA) susceptible isolates. Using DNA sequencing to screen for gene mutations conferring drug resistance may be feasible and use less time than using DST to detect resistance patterns.
Highlights
Multidrug-resistant tuberculosis (MDR-TB) is commonly found in Thailand especially in the public health region 5, the Western region of Thailand
As reported by the Global Tuberculosis Report 2018, MDR-TB was found in 82% of the people who showed resistance to treatment with rifampicin, the most effective first line drug [1]
Antimicrobial Susceptibility Testing (AST) is the conventional gold standard used to diagnose MDR-TB, but it causes delays in reporting of the resistance pattern when compared to the DNA sequencing method used to determine the mutation of resistance genes
Summary
Multidrug-resistant tuberculosis (MDR-TB) is commonly found in Thailand especially in the public health region 5, the Western region of Thailand. The most common gene mutations found using DNA sequencing were katG_Ser315Thr (70%), rpoB_Ser531leu (81%) and pncA_Ile31Thr (84%). Conclusion: Using DNA sequencing to screen for gene mutations conferring drug resistance may be feasible and use less time than using DST to detect resistance patterns. Thailand is one of the 14 countries of the world with a high rate of HIV-related TB infections and drug-resistant TB, verging on a Thai public health crisis with an estimated 130,000 TB cases (189 cases/100,000 population) in 2009 and 110,000 cases (159 cases/100,000 population) in 2012 [2]. Antimicrobial Susceptibility Testing (AST) is the conventional gold standard used to diagnose MDR-TB, but it causes delays in reporting of the resistance pattern when compared to the DNA sequencing method used to determine the mutation of resistance genes. The molecular test is based on the principle of nucleic acid
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