Abstract
Collagen have been interesting material for many utilization such as food, pharmaceutical and cosmetic in various products and target administration, consequently collagen should be prepared as well as type of application. The objective of this research is to prepare collagen from goat skin and investigate the character and their potency as an antioxidant. Kacang goat skin aged 2 years was used for collagen production. Small slice skin was extracted by curing with 0.1% (w/v) pepsin in acetic acid 0.5 M, for 24, 48, dan 72 h at 4°C. The variables observed were molecular weight by Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE), microstructure using scanning electron microscope, thermal stability by differential scanning calorimetric, and the antioxidant potency through 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition analysis. The result showed the molecular weight range from 25 kDa to 180 kDa, microstructure showed the collagen fibril crosslink, collagen start to denature at 62,28°C, highest dissolved with 1% NaCl concentration and has highest DPPH inhibition at 60 min after hydrolysis. In conclusion, kacang goat skin collagen prepared by pepsin in acetic acid.
Highlights
Collagen is one of the most important abundant single protein in the body of vertebrate animals because constituting 30% of total proteins was represented collagen (Bhatnagar et al 1999; Grover, Cameron, and Best 2012)
Type 1 collagen has many functions in the body, such as to biomaterial in wound dressing systems, in human used to implants of tissue engineering, and to drug delivery systems (Lee, Singla, and Lee 2001; (Friess, 1998)
Materials Indonesia Local kacang goat skin, sodium hydroxide and sodium chloride were purchased from Merck KgaA (Germany), acetic acid was purchased from SigmaAldrich (Germany), pepsin from porcine gastric mucosa was purchased from Merck (Germany), DPPH (1,1-diphenyl2picrylhydrazyl), methanol, sodium hydroxide was purchased from Merck Kga (Germany), and trichloroacetic acid (TCA)
Summary
Collagen is one of the most important abundant single protein in the body of vertebrate animals because constituting 30% of total proteins was represented collagen (Bhatnagar et al 1999; Grover, Cameron, and Best 2012). One of the type 1 collagen was many found in skins from animal tissues. Alternative of a raw material to production of collagen must be to find different sources. Study of collagen from goat skin is very limited. Object this study of collagen is using Indonesian local kacang goat skin which has a high population in Indonesian. Method of isolation collagen from skin was extraction with using chemical methods and enzymatic methods. The objective of this study was to isolation of collagen from new source with using Indonesian local kacang goat skin and extracted with enzymatic process. Enzyme used to extraction collagen is pepsin. The objective of this research is to prepare collagen from goat skin and investigate the character and their potency as an antioxidant. The variables observed were molecular weight by SDS PAGE, microstructure using scanning electron microscope, thermal stability by differential scanning calorimetric, and the antioxidant potency through DPPH inhibition analysis
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