Abstract
Background Escherichia coli sequence type 131 (ST131) is an important multidrug-resistant extraintestinal pathogen, which can cause many kinds of infections. Integrons may play a crucial role in the dissemination of antibiotic resistance genes. The purpose of this study was to characterize the prevelance of integrons among E. coli ST131 strains in China. Methods Eighty-three E. coli ST131 strains in China. E. coli ST131 strains in China. Results Overall, 26.5% (22/83) of the E. coli ST131 strains in China. dfrA17-aadA5 and aac(6′)-Ib-cr-cmlA5. Only one type of Pc promoter variant was detected among 22 integron-positive isolates (PcW). In vivo transfer of integron was successful for 9 of integron-positive E. coli ST131 strains in China. E. coli ST131 strains in China. Conclusions Our study showed a low prevalence of integrons was detected in E. coli ST131. Continued surveillance of this mobile genetic element should be performed to study the evolution of antibiotic resistance among E. coli ST131.E. coli ST131 strains in China. E. coli ST131 strains in China.
Highlights
Integrons are genetic elements found in bacteria, which can capture and express antimicrobial resistance gene cassettes [1]
Especially class 1 and 2 ones, have a worldwide distribution in Enterobacteriaceae [10, 11]. is has led to the speculation that integrons probably involve in the dissemination of antimicrobial resistance genes in E. coli sequence type 131 (ST131)
Class 2 and class 3 integrons were not detected in the study. e clinical characteristics of E. coli ST131 are showed in Table 1. e result of serotype revealed the integron-positive E. coli ST131 belonged to two types, and the most prevalence type was O25b (77.3%, 17/22)
Summary
Escherichia coli sequence type 131 (ST131) is an important multidrug-resistant extraintestinal pathogen, which can cause many kinds of infections. E presence and characterization of class 1, 2, and 3 integrons, as well as promotor of gene cassettes and other antimicrobial resistance genes, were detected by PCR and DNA sequencing. 26.5% (22/83) of the E. coli ST131 isolates carried class 1 integrons. One type of Pc promoter variant was detected among 22 integron-positive isolates (PcW). In vivo transfer of integron was successful for 9 of integron-positive E. coli ST131 isolates harboring resistance gene cassettes. Results of PFGE demonstrated that the integron-positive E. coli ST131 isolates were grouped into 12 different PFGE clusters. Our study showed a low prevalence of integrons was detected in E. coli ST131. Continued surveillance of this mobile genetic element should be performed to study the evolution of antibiotic resistance among E. coli ST131
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