Characterization of ingested MPs and their relation with growth parameters of endemic and invasive fish from a coastal wetland

Abstract

Microplastic (MP) contamination is a persistent and ubiquitous threat to aquatic ecosystems. This study quantifies MP ingestion by fish inhabiting the Anzali Wetland (Iran), a hotspot of biodiversity. Growth parameters have been monitored in endemic demersal fish (Caspian spined loach, Sabanejewia caspia), and invasive benthopelagic species (Prussian carp, Carassius gibelio) in the wetland and compared with their internal content of MPs. MPs were extracted from the gastrointestinal (GI) tracts following digestion of the samples in alkaline medium and observation of the extracts with microscopy (Scanning Electron Microscopy equipped with an Energy-Dispersive X-ray microanalyzer (SEM-EDS) and confocal Raman microscopy). A total of 84.6 % of the study fish (n = 26) were contaminated with MPs. Fibres were the only type of MPs found in the GI tracts, and these were mainly dark blue and made of polycarbonate and nylon in both investigated species. The mean numbers of MPs in the GI tracts of the carp and the loach were 3.6 and 3.7 respectively. MPs had smooth surfaces in most cases although some presented brittle, fragmented, and uneven surfaces and signs of degradation. The growth rates of Carassius gibelio and Sabanejewia caspia, measured with the b value (growth factor), were 2.91 and 2.15 respectively. Carassius gibelio can play a significant role in the transport of MPs to other aquatic organisms inhabiting the Anzali wetland, and hence can cause potential harm to them. Carassius gibelio MP contamination was more pronounced with increasing gut mass in older specimens. Due to the presence of MPs and in fish that can be consumed, there could be a trophic transfer to humans. Regarding Sabanejewia caspia, although not statistically significant, their uptake of MPs tends to increase in older specimens with smaller size and body weight. This can imply that MP pollution causes inappropriate conditions and results in negative growth. The findings of this work provide new insights into MP contamination in the Anzali wetland, specifically in endemic fish. These results will be important in conservation and management programs.