Characterization of induced pluripotent stem cell-derived megakaryocyte lysates for potential regenerative applications.

Anja Baigger,Rainer Blasczyk,Constanca Figueiredo,Denys Pogozhykh,Yuliia Yuzefovych,Dorothee Eicke

doi:10.1111/jcmm.13698

Anja Baigger, Rainer Blasczyk + Show 4 more

Open Access

https://doi.org/10.1111/jcmm.13698

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Abstract

Recently, platelet‐derived growth factors present in lysates became an extreme interest in the field of regenerative medicine such as in wound healing and as substitutes to foetal bovine serum in xeno‐free cell culture systems. However, the generation of such platelet lysates completely depends on the availability of platelet donors. In this study, the possibility to use in vitro‐generated megakaryocytes derived from induced pluripotent stem cells (iPSCs) as a cell source for typical platelet growth factors was investigated. Therefore, the presence and levels of those factors were characterized in in vitro‐produced megakaryocytes. In comparison with platelets, in vitro‐generated megakaryocytes showed a multifold increased content in transcript and protein levels of typical platelet growth factors including platelet‐derived growth factors (PDGFs), transforming growth factor (TGF)‐1β, vascular endothelial cell factor (VEGF)‐A, epidermal growth factor (EGF), insulin‐like growth factor (IGF)‐1 and tissue factor (TF). Hence, iPSC‐derived megakaryocytes may serve as an efficient cell source for a donor‐independent generation of growth factor‐rich lysates with a broad application potential in innovative cell culture systems and regenerative therapies.

Highlights

Megakaryocytes (MKs) are bone marrow residential platelet (PLT) precursors which release their progeny by cytoplasmic shedding into the sinusoidal vasculature
growth factors (GFs)-rich hPLs were considered of extreme relevance in regenerative medicine and stem cell therapies[3] as well as substitutes to foetal calf serum (FCS) in xeno-free cell culture systems.[4]
Platelet-derived growth factor (PDGF), transforming growth factor (TGF)-1b, vascular endothelial growth factor (VEGF)-A, epidermal growth factor (EGF), insulin-like growth factor (IGF)-1 and tissue factor (TF) have been described as relevant active factors contributing for the regenerative potential of hPLs.[3,4]

Summary

Introduction

Megakaryocytes (MKs) are bone marrow residential platelet (PLT) precursors which release their progeny by cytoplasmic shedding into the sinusoidal vasculature. KEYWORDS growth factors, megakaryocytes, platelet lysate, regenerative medicine

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