Characterization of Indigenous Rhizobacterial Isolates from Healthy Chilli Rhizosphere Capable of Inducing Resistance Against Anthracnose Disease (Colletotrichum Gloeosporioides).

Abstract

Antrachnose disease on chilli caused by Colletotrichum gloeosporioides is difficult to be controlled because the disease can be transmitted through the seeds, and has a high genetic diversity. One of promising alternative control is using biological control agents, such as groups of rhizobacteria. The objective of this research were : to characterize the morphology, physiology and molecular of selected rhizobacterial isolates, which were capable of controlling the anthracnose disease and to enhance the growth and yield chilli. Three rhizobacterial isolates (B1.37, B2.11 and P1.31) were used. These isolates were indentified based on morphology (colony form, elevation, edge, and color), physiology (gram tes, the production of hormone IAA, chitinase enzyme, hydrogen cyanide, and solvents phosphate) and molecular. The isolates were identified by using 16S rRNA sequencing. The results indicated that isolate B1.37 belonged to species of Bacillus cereus strain ML 267, isolate B2.11 belonged to Bacillus cereus strain LH8 and isolate P1.31 belonged to Chryseobacterium gleum strain NBRC 15054.