Abstract
BackgroundCheilanthes tenuifolia, a member of the Pteridaceae family, is an evergreen and small fern could be abundant of bioactive compounds. The present study was designed to investigate its many therapeutic properties and isolation of bioactive compounds from extracts of Cheilanthes tenuifolia.MethodsThe dried coarse plant powder was extracted with methanol and dried with rotary evaporator. The extract was further partitioned according to the increasing polarity: N-hexane < chloroform < ethyl-acetate < methanol by modified Kupchan method. Then each extract fractions were investigated for their pharmacologic properties. Compounds were isolated from n-hexane fraction through column chromatography, followed by TLC and structure was determined by analysis of sample using 1H-NMR and matched with published phytochemistry report.ResultsMethanol fraction of Cheilanthes tenuifolia showed highest amount of total phenol content (11.32 ± 0.28 mg/gm GAE) followed by chloroform fraction (9.71 ± 0.31 mg/gm GAE) > N-hexane fraction (6.69 ± 0.67 mg/gm GAE) > ethyl acetate fraction (5.36 ± 0.54 mg/gm GAE). The methanol fraction of Cheilanthes tenuifolia possessed highest amount (7.11 ± 0.52 mg/gm QE) of total flavonoid content. Our present study indicates that methanol extract was most potent (IC50 = 9.926 μg/ml) inhibitor of DPPH free radicals. In brine shrimp lethality bio assay, all the extracts showed dose dependent increment of mortality and chloroform extract was found most cytotoxic (LC50 = 34.493 μg/ml) compared to other plant extracts. The chloroform fraction of Cheilanthes tenuifolia was most potent in terms of thrombolytic activity. A compound was isolated (CT-2) using column chromatography followed by TCL and PTLC (35% pet ether in CHCl3) and analyzed by 1H-NMR. The structure of stigmasterol was confirmed by comparing the 1H-NMR data with previously published phytochemistry report.ConclusionCheilanthes tenuifolia could be a potential candidate for bioactive compounds and further studies on isolation and characterization of its bioactive compounds are highly required.
Highlights
Plants, their whole parts or derivatives, most important natural resource are used for therapeutic benefits
Phytochemical groups Methanolic extract of whole plant of Cheilanthes tenuifolia was successively partitioned with N-hexane, chloroform and ethyl-acetate according to modified Kupchan partition method [11]
Our current study suggests that Cheilanthes tenuifolia possess very poor total antioxidant capacity
Summary
Their whole parts or derivatives, most important natural resource are used for therapeutic benefits. It has been assumed that approximately 250 species of medicinal plants are employed for the preparation of traditional medicines which is the 50 % of total species of plants grown in Bangladesh [5]. Bulk of these plant kingdoms has not yet undergone extensive chemical, pharmacological, and toxicological research to investigate their bioactive compounds. Cheilanthes tenuifolia, a member of the Pteridaceae family, is an evergreen and small fern could be abundant of bioactive compounds. The present study was designed to investigate its many therapeutic properties and isolation of bioactive compounds from extracts of Cheilanthes tenuifolia
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
