Characterization of Immature Bamboo (Phyllostachys nigra) Component Changes with Its Growth via Heteronuclear Single-Quantum Coherence Nuclear Magnetic Resonance Spectroscopy.

Abstract

A 6.2 m high immature bamboo (Phyllostachys nigra) was divided into seven fractions. The bamboo cell walls and lignin samples from young to old were characterized by 1H-13C correlation heteronuclear single-quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectroscopy both qualitatively and semiquantitatively. Mature bamboo and bamboo shoot samples were used as comparison references. HSQC-NMR analysis proved that cellulose and arabinoxylan have already deposited in bamboo shoot, and cellulose amount increased during growth. Lignin side chain linkage formation started from β-ether (β-O-4), then phenylcoumaran (β-5), and finally resinol (β-β). Ferulic acid and p-coumaric acid (pCA) were formed at the earlier stages in the immature bamboo, and the pCA proportion decreased throughout the lignification process. We propose that the bamboo lignification process is distinct from both woody and other herbaceous plants, where syringyl units deposited at the early stage and polymerized with the β-O-4 linkage. Then guaiacyl units formed gradually, and finally, p-hydroxyphenyl units formed.