Characterization of IgE antibody binding profiles of sera from patients with atopic dermatitis to allergens of the domestic mites Dermatophagoides pteronyssinus and Euroglyphus maynei, using enhanced chemiluminescent immunoblotting.

Abstract

A rapid, sensitive, chemiluminescent immunoblotting method was used to identify IgE-binding components of Dermatophagoides pteronyssinus and Euroglyphus maynei that were recognized by sera of 16 D. pteronyssinus-sensitive patients with atopic dermatitis. Some 28 components (14-200 kD) were found in the D. pteronyssinus extract, and sera bound between 1 and 13 of them (mean 6.0). At least one of four components (97, 28, 24 and 14 kD) were bound by 14/16 sera. E. maynei had 25 components (14-227 kD), 13 in common with those of D. pteronyssinus by molecular size. Sera bound between 1 and 11 of them (mean 5.2) and 14/16 sera bound 1 of 38 kD (unique to E. maynei) and/or 1 of 175 kD. There were positive correlations between the number of D. pteronyssinus and E. maynei components bound and anti-D. pteronyssinus and E. maynei serum IgE values (p less than 0.001 in both cases) and between the number of D. pteronyssinus and E. maynei components bound and total serum IgE values (p less than 0.01 in both cases). These results demonstrate a very considerable diversity of IgE antibody responses to domestic mite allergens amongst patients with atopic dermatitis.