Characterization of hydroxyapatite-containing alginate–gelatin composite films as a potential wound dressing

Abstract

In this study, hydroxyapatite (HA)-containing alginate–gelatin films were prepared by solution casting method by blending alginate (A) and gelatin (G) solutions, followed by crosslinking with calcium chloride. HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined. The release of tetracycline hydrochloride (TH), selected as a model drug, from the prepared films was studied. It was observed that the swelling ratio and weight loss of the films decreased as the amounts of alginate and HA increased. Scanning electron microscopy analysis indicated that as the amount of HA in the films increased, the film surface becomes rougher. The mechanical properties of the films were affected by the amount of HA and the A:G ratio. Incorporation of HA increased the thermal stability of films. The amount of TH released from the films within 15 min decreased as the amounts of alginate and hydroxyapatite increased. It was found that films containing TH showed slightly higher antimicrobial activity against Staphylococcus aureus than Escherichia coli.