Abstract
Apple chlorotic leaf spot trichovirus is considered one of the most economically important viruses affecting both pome and stone fruit species. High biological and molecular variability is known to occur among ACLSV isolates of different countries. Occurrence of ACLSV on apple in Hungary is known since the age of ‘60s. Severe symptoms, not characteristic for any known virus disease of stone fruits, were observed on peach in 1974 and on wild cherry in 1982. Later it was suspected that those symptoms were caused by atypical isolates of ACLSV. Therefore the above virus isolates were studied and compared to ACLVS isolates from apple. IC-RT-PCR, Western blot analyses as well as on cloning and sequencing of PCR products were performed. On the basis of electrophoretic mobility of the coat protein, which ranged between 19.7 and 22.7 kDa, Hungarian isolates could be classified, with different percentages, into three distinct groups. Six of 12 isolates belonged to Cis, 5 belonged to Bit and one mixed infection (Cen+Bit) was found. Virus cDNA was amplified by IC-RT-PCR. Amplified fragments of two apple, one peach and two cherry isolates were cloned, sequenced and compared. Homology of the compared isolates ranged between 81-94%.
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