Abstract
Background Analysis of human monoclonal antibodies (mAbs) developed from HIV-1 infected donors have enormously contributed to the identification of neutralization sensitive epitopes on the HIV-1 envelope glycoprotein. We have isolated 3 anti-V3 mAbs 277, 903 and 904 by hybridoma technology from EBV transformed B cells of HIV-1 seropositive drug naive patients. The ELISA binding revealed a subtype-C and subtype-A specific binding of antibody 277 and 903 while 904 exhibited cross reactivity also with subtype-B V3. Epitope mapping of mAbs with overlapping V3 peptides showed exclusive binding to V3 crown. The antibodies displayed high and low neutralizing activity against 2/5 tier 1 and 1/6 tier 2 viruses respectively.
Highlights
Analysis of human monoclonal antibodies developed from Human Immunodeficiency Virus type-1 (HIV-1) infected donors have enormously contributed to the identification of neutralization sensitive epitopes on the HIV-1 envelope glycoprotein
Characterization of human anti-V3 monoclonal antibody 904 isolated from an Indian clade C Human Immunodeficiency Virus type-1 (HIV-1) infected donor
Analysis of human monoclonal antibodies developed from HIV-1 infected donors have enormously contributed to the identification of neutralization sensitive epitopes on the HIV-1 envelope glycoprotein
Summary
Characterization of human anti-V3 monoclonal antibody 904 isolated from an Indian clade C Human Immunodeficiency Virus type-1 (HIV-1) infected donor. From Frontiers of Retrovirology: Complex retroviruses, retroelements and their hosts Cambridge, UK. From Frontiers of Retrovirology: Complex retroviruses, retroelements and their hosts Cambridge, UK. 16-18 September 2013
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