Abstract
The composition of cytoplasmic and nuclear histone H3.1 predeposition complexes was analyzed in this study. These complexes are used for the building of nucleosome, a basic unit of chromatin. Core histones H3.1, H4, histone chaperones and histone acetyl-transferases were identified using mass spectrometry in both nuclear and cytoplasmic complexes. Importin-beta was revealed as specific component of cytoplasmic complex and large subunit of histone chaperone CAF-1 p150 as specific component of nuclear complex.
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