Abstract

A lipoprotein species with ultracentrifugal flotation rates ( F 1.20 0 9–28) intermediate to high density lipoproteins (HDL, F 1.20 0 0–9) and low density lipoproteins (LDL, F 1.20 0 28–56) found in the plasma of certain pedigreed baboons fed an atherogenic diet was studied by gradient gel electrophoresis (GGE) and ultracentrifugal techniques. These lipoproteins were found to be heterogeneous in size (125–220 Å) and hydrated density (1.028–1.080 g/ml). The major apolipoprotein in all density subfractions of the F .20 o 9–28 lipoproteins exhibited the molecular weight (2.8 × 10 4 daltons) and immunochemical properties of apolipoprotein A-1 (apoA-I). Protein corresponding to apolipoprotein E (apoE, 3.5 × 10 4 daltons),was observed primarily in the less dense subspecies of F 1.20 0 9–28 lipoproteins. Some low molecular weight (1.8 × 10 4, 1.3 × 10 4, and 1.1 × 10 4 daltons) apolipoproteins were also detected. At low serum F 1.20 0 9–28 lipoprotein concentrations, only the smaller, more dense, protein-rich species were present; at higher F 1.20 0 9–28 concentrations, the larger, less dense species were observed in addition to the small species. The HDL of pedigreed baboons in families with and without serum F 1.20 0 9–28 lipoproteins were also characterized. The HDL of both groups of progeny consisted of a similar set of 5 subpopulations designated HDL-I through HDL-V determined by GGE. HDL-I, consisting of material 100–125 Å in size, was the major HDL subpopulation. ApoA-I was the major protein moiety in all HDL subpopulations; none contained apoE. Baboons in families with F 1.20 0 9–28 lipoproteins had more HDL-I (292 ± 80 mg/dl vs. 235 ± 55 mg/dl) and less HDL-II (86 ± 22 mg/dl vs. 135 ± 34 mg/dl) than baboons in families without F 1.20 0 9–28 lipoproteins; both groups showed identical total HDL concentrations (446 ± 90 mg/dl and 444 ± 49 mg/dl, respectively). Among those baboons in families with F 1.20 0 9–28 lipoproteins, there was an inverse correlation between F 1.20 0 9–28 concentration and total HDL, HDL-I and HDL-II concentrations, indicating a possible metabolic relationship between these HDL subpopulations and the F 1.20 0 9–28 species.

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