Abstract
The bovine gene map is relatively void of markers compared to the human, mouse and rat gene maps; this is mainly due to the difficulty in identifying the bovine chromosomes (FRIES et al. 1989). The localizations of loci for productive characters and disease resistance or susceptibility are of major interest for their potential manipulation in breeding programs. Cattle gene mapping can be performed by interspecific hamster-bovine cell hybrids. Since the bovine chromosomes are selectively lost, it is possible to establish an association between specific markers and the chromosomes retained ( HEUERTZ and HORS-CAYALA 1978). Panels of hamsterbovine cell hybrids have been produced in a few laboratories, but in most cases they have only partially been cytogenetically characterized as most of the synteny groups have not been assigned to a specific bovine chromosome (DAIN et al. 1984; WOMACK and MOLL 1986). In order to identify the bovine chromosomes in hamster-bovine hybrids, we combined standard banding techniques with in situ hybridization. Moreover, a protocol for interphase analysis of hybrid cell populations was developed which allowed the rapid selection of clones to be further characterized.
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