Characterization of GSK-M, a glycogen synthase kinase from rat skeletal muscle

Abstract

A form of glycogen synthase kinase designated GSK-M 3 3 Abbreviations used: UDP-Glc, uridine diphosphoglucose; Glc-6-P, glucose 6-P; GS, glycogen synthase; GSK, glycogen synthase kinase; GSK-M, rat skeletal muscle glycogen synthase kinase; GSK-3, glycogen synthase kinase 3 also known as kinase F A; F A, ATP-Mg-dependent protein phosphatase activating factor; GSK-4, glycogen synthase kinase 4; CB peptide, peptide obtained after fragmentation with CNBr; SDS, sodium dodecyl sulfate; TFA, trifluoroacetic acid; TPCK, l-1-(tosylamido)-2-phenylethyl chloromethyl ketone. was purified 4000-fold from rat skeletal muscle by phosphocellulose, Affi-Gel blue, Sephacryl S-300 and carboxymethyl-Sephadex column chromatography. Separation of GSK-M from the catalytic subunit of the cAMP-dependent protein kinase was facilitated by converting the catalytic subunit to the holoenzyme form by addition of the regulatory subunit prior to the gel filtration step. GSK-M had an apparent M r 62,000 (based on gel filtration), an apparent K m of 11 ν m for ATP, and an apparent K m of 4 μM for rat skeletal muscle glycogen synthase. The kinase had very little activity with 0.2 m m GTP as the phosphate donor. Kinase activity was not affected by the addition of cyclic nucleotides, EGTA, heparin, glucose 6-P, glycogen, or the heat-stable inhibitor of cAMP-dependent protein kinase. Phosphorylation of glycogen synthase from rat skeletal muscle by GSK-M reduced the activity ratio (activity in the absence of Glc-6-P/activity in the presence of Glc-6-P × 100) from 90 to 25% when approximately 1.2 mol of phosphate was incorporated per mole of glycogen synthase subunit. Phosphopeptide maps of glycogen synthase obtained after digestion with CNBr or trypsin showed that this kinase phosphorylated glycogen synthase in serine residues found in the peptides containing the sites known as site 2, which is located in the N-terminal CNBr peptide, and site 3, which is located in the C-terminal CNBr peptide of glycogen synthase. In addition to phosphorylating glycogen synthase, GSK-M phosphorylated inhibitor 2 and activated ATP-Mg-dependent protein phosphatase. Activation of the protein phosphatase by GSK-M was dependent on ATP and was virtually absent when ATP was replaced with GTP. GSK-M had minimal activity toward phosphorylase b, casein, phosvitin, and mixed histones. These data indicate that GSK-M, a major form of glycogen synthase kinase from rat skeletal muscle, differs from the known glycogen synthase kinases isolated from rabbit skeletal muscle.