Abstract
Globodera ellingtonae was originally described from populations collected in the United States. In the original description, ribosomal DNA loci from Globodera sp. collected in Chile and Argentina were similar to G. ellingtonae, suggesting this nematode originated in this region of South America. In an effort to find additional populations of G. elllingtonae, collection trips were conducted in 2017 and 2020 in the Antofagasta and Arica y Parinacota Regions in Northern Chile, respectively. Globodera sp. were more prevalent in Antofagasta (17 samples collected, 53% positive for Globodera sp.) than in Arica y Parincota (16 samples collected, 13% positive for Globodera sp.). The genomes of single cysts (N ≥ 3) from four fields were sequenced. Additionally, the genomes of the G. ellingtonae population from Oregon and a Globodera sp. population originally collected in Antofagasta Region but maintained in culture in France were also sequenced. Based upon a HSP90 sequenced data mined from WSG data, all of the populations from the Antofagasta Region were G. ellingtonae and grouped in a monophyletic clade. A population collected from the Arica y Parincota Region was identified as G. rostochiensis based upon HSP90 data. Genome-wide SNP patterns of the G. ellingtonae populations showed strong clustering based on geographic location indicating that G. ellingtonae has high genetic diversity within Chile. A phylogenetic tree derived from 168,354 binary SNPs in the nuclear genome showed separate but distinct clustering of the Oregon population and the population from Antofagasta maintained in France. The Oregon G. ellingtonae population subtended the Chilean clades and placed on a long branch representing approximately twice the genetic variation observed among all Chilean G. ellingtonae populations. The possibility remains that G. ellingtonae from Chile may be sufficiently diverged to constitute a new species from G. ellingtonae originally described from a population collected in Oregon.
Highlights
Globodera ellingtonae (Handoo et al, 2012) was first detected, and subsequently described from populations collected in Oregon and Idaho (Handoo et al, 2012)
A phylogenetic analysis based on the ITS1 and -2 rDNA indicated that the G. ellingtonae population from Oregon was most similar to Globodera sp. populations collected in Argentina and Chile
A multigene phylogeny based upon 6,933 bp encompassing 11 genes, further confirmed the closer relationship of G. ellingtonae to G. rostochiensis and the G. tabacum complex than to G. pallida (Zasada et al, 2015)
Summary
Globodera ellingtonae (Handoo et al, 2012) was first detected, and subsequently described from populations collected in Oregon and Idaho (Handoo et al, 2012). A subsequent study on a G. ellingtonae population from Argentina obtained similar results based on HSP90 and ITS regions (Lax et al, 2014) In these analyses G. ellingtonae was more closely related to G. rostochiensis and the G. tabacum complex than to G. pallida. There are sequences for four populations of G. ellingtonae available in Genbank from the United States, Chile, and Argentina (Handoo et al, 2012; Lax et al, 2014; Skantar et al, 2011) This is just a handful of data compared to what is available for G. rostochiensis and G. pallida (Subbotin et al, 2020). Utilizing WGS data, we explored population genetics among the G. ellingtonae populations and compared informative gene regions of these populations to available data
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
