Characterization of genotypes for Burkholderia cepacia complex strains isolated from patients in hospitals of the Russian federation

Abstract

Eighty-eight cultures of microorganisms classed as part of the Burkholderia cepacia complex (Bcc) during initial identification were analyzed by multilocus sequencing (multilocus sequence typing, MLST). Thirteen genotypes (sequence type, ST) were detected; nine of them (708, 709, 710, 711, 712, 714, 727, 728, 729) were identified for the first time. Two new alleles for the gene trpB (357, 358) and one of the genes atpD (306) and gltB 9352 were detected and registered. It was found that strains of two genotypes (711, 712) belong to the species B. multivorans, one (ST102) to B. contaminans, one (ST51) to B. stabilis, and one (ST729) to B. vietnamiensis. Most strains of the sample representing eight genotypes (208, 241, 728, 727, 708, 709, 710, 714) belong to the species B. cenocepacia. The identified genotypes differ in global extent: four genotypes (51, 102, 208, 241) have intercontinental distribution, and one (712) has intracontinental distribution. It is shown that strains causing nosocomial infections in most cases refer to genotypes 728 and 708. Genotype 709 detected in strains isolated from patients in seven federal districts (FDs) of Russia should be recognized as epidemiologically significant for patients with cystic fibrosis. The Bcc strains of genotypes 241 (B. cenocepacia) and 729 (B. vietnamiensis) were isolated from the patients of the Far Eastern FD. They are not typical for the other FDs of Russia. The possibility of concomitant infection in cystic fibrosis patient with two genotypes (709, 708), that is, are epidemiologically significant and nosocomial was indicated. The longterm persistence of a single genotype strain in the organism of patients with cystic fibrosis was shown both for Bcc species B. cenocepacia (ST 709) and for B. multivorans (ST712). The possibility of transfection of the Bcc strain typical for a nosocomial environment to patients with cystic fibrosis during surgery was observed.