Abstract
Glycolytic metabolism is closely involved in physiological homeostasis and pathophysiological states. Among glycolytic enzymes, phosphoglycerate mutase (PGAM) has been reported to exert certain physiological role in vitro, whereas its impact on glucose metabolism in vivo remains unclear. Here, we report the characterization of Pgam1 knockout mice. We observed that homozygous knockout mice of Pgam1 were embryonic lethal. Although we previously reported that both PGAM-1 and -2 affect global glycolytic profile of cancers in vitro, in vivo glucose parameters were less affected both in the heterozygous knockout of Pgam1 and in Pgam2 transgenic mice. Thus, the impact of PGAM on in vivo glucose metabolism is rather complex than expected before.
Highlights
Glucose is utilized as energy source, from bacterias to higher eukaryotes
As it has been demonstrated that the overexpression of either phosphoglycerate mutase (PGAM) isoform confers similar physiological impact [11,12,13], we evaluated the in vivo parameters of glucose metabolism in Pgam2-Tg mice, in which a Pgam2-FLAG transgene is driven by the CAG promoter
We reported on the profiles of in vivo glucose parameters in genetic models of glycolytic enzyme PGAM
Summary
Glycolysis provides energy supply in the form of ATP through oxidation of carbon atoms in glucose. As it supports the synthesis of macromolecules [1], glycolysis is evolutionally conserved as one of vitally essential metabolisms. Dysregulation in glycolysis, either the pathological enhancement or impairment, is closely related to human disease states. In humans and other vertebrates, glucose is delivered to various tissues through circulating blood. Even during prolonged fasting (no calorie feeding), blood sugar levels are minimally maintained, which enables various tissues and cells, e.g. neurons in brain, to utilize glucose from circulating blood.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
