Abstract
Pectobacterium carotovorum subsp. carotovorum is a well-known plant pathogen that causes severe soft rot disease in various crops, resulting in considerable economic loss. To identify pathogenicity-related factors, Chinese cabbage was inoculated with 5314 transposon mutants of P. carotovorum subsp. carotovorum Pcc21 derived using Tn5 transposon mutagenesis. A total of 35 reduced-virulence or avirulent mutants were isolated, and 14 loci were identified. The 14 loci could be functionally grouped into nutrient utilization (pyrD, purH, purD, leuA and serB), production of plant cell-wall-degrading enzymes (PCWDEs) (expI, expR and PCC21_023220), motility (flgA, fliA and flhB), biofilm formation (expI, expR and qseC), susceptibility to antibacterial plant chemicals (tolC) and unknown function (ECA2640). Among the 14 genes identified, qseC, tolC and PCC21_023220 are novel pathogenicity factors of P. carotovorum subsp. carotovorum involved in biofilm formation, phytochemical resistance and PCWDE production, respectively.
Highlights
Pectobacterium carotovorum subsp. carotovorum causes soft rot disease in cabbage, potato, onion, radish and other crops during cultivation, transportation and storage, resulting in considerable economic damage
After successfully invading a plant, it resides in the intercellular space or vascular tissue, where it produces plant cell-wall-degrading enzymes (PCWDEs) in a disease-promoting environment (Perombelon, 2002; Toth et al, 2003)
To isolate novel pathogenicity-related genes, we used a simple mutagenesis system based on a transposon– transposase synaptic complex
Summary
Pectobacterium carotovorum subsp. carotovorum causes soft rot disease in cabbage, potato, onion, radish and other crops during cultivation, transportation and storage, resulting in considerable economic damage. Plasmid by self-ligation from Pcc21 Tn5 mutant, pyrD : : Tn5, Kanr Plasmid by self-ligation from Pcc21 Tn5 mutant, purH : : Tn5, Kanr Plasmid by self-ligation from Pcc21 Tn5 mutant, purD : : Tn5, Kanr Plasmid by self-ligation from Pcc21 Tn5 mutant, leuA : : Tn5, Kanr
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