Abstract
The objectives of this study were to isolate and identify garlic endophytes, and explore the characteristics of dominant strains. Garlic endophytes were studied through phenotypical characterization and comparative sequence analysis of 16S rDNA based on culture‐dependent approaches. Representative strains inferred from 16S rDNA sequencing were selected for further identification by gyrA and rpoB gene loci and phylogenetic analysis based on concateneted house‐keeping sequences. Seven kinds of Bacillus were found from garlic and black garlic, respectively. Further studies demonstrated that the total bacteria and endophytes showed a sharp decrease firstly, followed by a rapid rise, then maintained at a certain level, and finally slowed down during the black garlic processing. B. subtilis, B. methylotrophicus, and B. amyloliquefaciens were the dominant strains. The selected strains were capable of fermenting glucose, lactose, sucrose, and garlic polysaccharide to produce acid but no gas, with a strong ability of heat resistance. The results indicated that there were a certain number of garlic endophytes during the black garlic processing, and Bacillus was the dominant strains under the conventional culture‐dependent methods. This report provided useful information for the presence and type of garlic endophytes during the black garlic processing, which were of great significance to study the formation mechanism and quality improvement of black garlic in the future, as well as the security of garlic powder.
Highlights
Endophytes can be defined as those microbes that colonize the internal tissues of healthy plants, showing no obvious external sign of infection or negative effect on their host
The results provided a insight into the presence and type of garlic endophytes, which contributed to the further research of black garlic formation mechanism and quality improvement, and clarified the source of Bacillus in garlic powder for the security
On the 16S rDNA, gyrA, gyrB, and rpoB genes were shown in Table 5, as well as a portion of the 16S rDNA alone identification
Summary
Endophytes can be defined as those microbes that colonize the internal tissues of healthy plants, showing no obvious external sign of infection or negative effect on their host. There have been a hundred years of history on the research of endophytes, with endophytes found in almost every plant studied (Ryan, Germaine, Franks, Ryan, & Dowling, 2008). Plant endophytes which coexist with host plants for a long term can produce a series of the same bioactive secondary metabolites as the host plants, such as antitumor bioactive substances, with great potential for medical, agricultural, and industrial exploitation (Kim et al, 2007). Garlic has a wide range of purposes on account of high nutritional value and unique flavor, regarded as one of the daily best healthy food, as demonstrated
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
