Characterization of Garden Cress Mucilage and its Prophylactic Effect Against Indomethacin-Induced Enter-Colitis in Rats

Abstract

The utilization of industrial waste such as oil industry waste in the production of natural nutraceuticals is a very beneficial issue. So, the current study was established to assess the characteristics of the mucilage extracted from garden cress seeds meal and evaluate its efficacy in the protection against enter-colitis. A defatted powder meal of garden cress seeds was dissolved in distilled water to extract the mucilage either by heating at 80°C or by ultrasonication. Functional and chemical characteristics of the two types of mucilage were assessed. Rats received oral administration of the mucilage extracted by ultrasonication, which afforded the most promising antioxidant activity, for two weeks before and during the 7 days of oral administration by indomethacin (6 mg/kg/day). Compared with the rats injected with indomethacin without the pre-treatment with the mucilage, rats administrated with the mucilage showed a decrease in the erythrocyte sedimentation rate (ESR), intestinal tumor necrosis factor-α (TNF-α), and plasma lactate dehydrogenase (LDH) activity. Also, rats administered with the mucilage recorded lower intestinal malodealdehyde (MDA) and higher intestinal reduced glutathione (GSH) compared to those of rats injected with indomethacin without the pre-treatment with the mucilage. It can be concluded that the mucilage extracted from garden cress seeds meal can be considered as a natural nutraceutical with potent antioxidant activity exhibiting protective effect against enter-colitis.