Characterization of Fungal Community Composition in the Rhizosphere Soil of Actinidia chinensis Planch Accompanied by Root Rot

Abstract

Nowadays, with the development of Actinidia chinensis Planch cultivation, occurrence of Actinidia chinensis Planch root rot disease have increasingly affected the yield and quality of Actinidia chinensis Planch which caused by many kinds of fungal communities. The aim of this paper is to study fungal community structure in rhizosphere soil of Actinidia chinensis Planch infected and non- infected by root rot disease, and to look for monitoring of Actinidia chinensis Planch root rot disease and green ecological control. The rhizosphere soil samples of Actinidia chinensis Planch infected and non-infected by root rot disease were collected respectively in western mountain areas of Huangyan, Taizhou city, Zhejiang province,China, which is the one of the largest Actinidia chinensis Planch planting regional of Zhejiang province. The fungal community structure of rhizosphere soil of Actinidia chinensis Planch were determined by high throughput sequencing techniques. Compared with non-infected soil, the Chao1 indexes of fungal in rhizosphere soil of Actinidia chinensis Planch infected by root rot disease were decreased with significant level. At phylum level, the relative abundance of some dominant fungal in rhizosphere soil of Actinidia chinensis Planch infected by root rot disease were lower than those of non-infected with significant level(P<0.01), such as Basidiomycota, Fungi-unclassified and Zygomycota, while the relative abundance of Ascomycota in rhizosphere soil of Actinidia chinensis Planch infected by root rot disease were higher than those of non-infected with significant level(P<0.01). At genus level, the relative abundance of some dominant fungal in rhizosphere soil of Actinidia chinensis Planch infected by root rot disease were higher than those of non-infected with significant level, such as Gibberella, Nectria, Fusarium, Pseudogymnoascus and Hypocrea, while the relative abundance of some fungal in rhizosphere soil of Actinidia chinensis Planch infected by root rot disease were lower than those of non-infected with significant level(P<0.01), including Agaricomycetes-unclassified, Cladorrhinum, Trechispora, Ophiocordyceps, Acremonium, Ascobolus and Mortierella. It is conclude that in rhizosphere soils of Actinidia chinensis Planch infected with root rot disease, At the phylum and genus levels, great variations in the abundance of the dominant fungal have been confirmed. Actinidia chinensis Planch root rot disease is closely related to the structure of rhizosphere fungi community, and the changes of the balance and diversity of soil fungi is the major cause of Actinidia chinensis Planch root rot disease. The finding could provide some theoretical reference for controlling Actinidia chinensis Planch root rot disease in Zhejiang Province.