Characterization of fluorescent dissolved organic matters in metalworking fluid by fluorescence excitation-emission matrix and high-performance liquid chromatography

Abstract

In recent years, precise environment supervision has gradually become vital in water pollution control, which requires the clear identification of dissolved organic matters (DOM) in wastewater. Metalworking fluid (MWF) is a type of wastewater with high toxicity. Over ten million m3 of MWF is discharged per year. However, its DOM characteristics have not yet been systematically investigated. Therefore, in this study, the fluorescent DOM (FDOM) of MWF was firstly characterized by excitation-emission matrix-parallel factor analysis (EEM-PARAFAC) and high-performance liquid chromatography (HPLC). Three fluorescent components (C1-C3) of the MWF from three metalworking plants (BO, TH, and YD) were identified. The peaks measured for C1 and C3 were attributed to tryptophan-like (Peak T) and humic/fulvic acid-like (Peak A + C) peaks, respectively, and the peaks at C2 were identified as humic-like (Peak A + M) or tryptophan-like (Peak T) peaks. There were differences in the C2 and C3 components of MWF from the three metalworking plants. The FDOM of MWF from the three metalworking plants exhibited similar polarity, but different apparent molecular weight distributions. In addition, the highest intensities of the three fluorescent peaks were sensitive to variations in the pH, humic acid (HA) concentrations, and metal ion levels (Cu2+, Fe3+, and Ni2+). The findings of this study not only indicate the FDOM characteristics of MWF, but also provide a promising method and valuable guidance for the practical monitoring of MWF in natural water bodies.