CHARACTERIZATION OF FLAVONOIDS ISOLATED FROM Trigonellafoenum-graecum USING UV SHIFT REAGENT

Abstract

The biggest challenge in utilizing plant secondary metabolites lies in extraction, isolation and characterization using available analytical techniques. UV absorption is an excellent technique for the identification and characterization of phenolic compounds. Phyto compounds characterization using UV absorption spectrum is very helpful and it gives quick results which can be interpreted easily, wherein preparation of the sample for analysis involves no difficulty. In this study, we have identified and characterized 2 flavonoids, kaempferol 3-o rutinoside and chrysoreol using UV shift reagents, isolated from Trigonella foenumgraecum methanolic seed extract. The same was confirmed by reference Mass and NMR spectral results reported in the literature. Characterization using UV-visible spectrometry is a powerful technique that can be employed for phytochemical identification and characterization purposes with great leverage when sample isolated is less.