Abstract
BackgroundCanine and human osteosarcomas (OS) are notably similar and have a high rate of metastasis. There is a poor understanding of the tumor development process, predisposing causes, and varying levels of aggression among different cell lines. By characterizing newly developed canine osteosarcoma cell lines, treatments for people and pets can be developed. Of the seven subtypes of OS, three are represented in this group: osteoblastic (the most common), fibroblastic, and giant cell variant. To our knowledge, there are no other giant cell variant canine OS cell lines in the published literature and only one canine fibroblastic osteosarcoma cell line. Understanding the differences between the histologic subtypes in dogs will help to guide comparative research.ResultsAlkaline phosphatase expression was ubiquitous in all cell lines tested and invasiveness was variable between the cell lines tested. Invasiveness and oxidative damage were not correlated with in vivo growth rates, where TOT grew the fastest and had the higher percentage of mice with metastatic lesions. TOL was determined to be the most chemo-resistant during cisplatin chemotherapy while TOM was the most chemo-sensitive.ConclusionsFurther comparisons and studies using these cell lines may identify a variety of characteristics valuable for understanding the disease process and developing treatments for osteosarcoma in both species.Some of this data was presented as a poster by KMF at the August 5th, 2017 National Veterinary Scholars Program in Bethesda, MA.Characterization of 5 newly generated canine osteosarcoma cell lines.Kelli Franks, Tasha Miller, Heather Wilson-Robles.
Highlights
Canine and human osteosarcomas (OS) are notably similar and have a high rate of metastasis
Cell proliferation rates varied among the different cell lines A 72-h proliferation assay was performed and yielded doubling times for each cell line
Alkaline phosphatase stain (ALP) staining varied between cell lines ALP staining performed on each cell line determined the percent of ALP positive cells present in each cell line
Summary
Canine and human osteosarcomas (OS) are notably similar and have a high rate of metastasis. The lack of a tumor microenvironment and tumor associated inflammation can alter surface marker expression as well as biologic pathways [1, 2]. These changes become more significant with the continued passaging of cells over time. Developed cell lines have undergone fewer passages and more closely resemble the primary tumor from which they were derived. These newly generated cell lines have the potential to add much needed variety to in vitro research and the body of literature available for a given tumor type
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